A new measure for community structures through indirect social connections

Based on an expert systems approach, the issue of community detection can be conceptualized as a clustering model for networks. Building upon this further, community structure can be measured through a clustering coefficient, which is generated from the number of existing triangles around the nodes over the number of triangles that can be hypothetically constructed. This paper provides a new definition of the clustering coefficient for weighted networks under a generalized definition of triangles. Specifically, a novel concept of triangles is introduced, based on the assumption that, should the aggregate weight of two arcs be strong enough, a link between the uncommon nodes can be induced. Beyond the intuitive meaning of such generalized triangles in the social context, we also explore the usefulness of them for gaining insights into the topological structure of the underlying network. Empirical experiments on the standard networks of 500 commercial US airports and on the nervous system of the Caenorhabditis elegans support the theoretical framework and allow a comparison between our proposal and the standard definition of clustering coefficient

Structural Bounds on the Dyadic Effect

In this paper we consider the dyadic effect introduced in complex networks when nodes are distinguished by a binary characteristic. Under these circumstances two independent parameters, namely dyadicity and heterophilicity, are able to measure how much the assigned characteristic affects the network topology. All possible configurations can be represented in a phase diagram lying in a two-dimensional space that represents the feasible region of the dyadic effect, which is bound by two upper bounds on dyadicity and heterophilicity. Using some network's structural arguments, we are able to improve such upper bounds and introduce two new lower bounds, providing a reduction of the feasible region of the dyadic effect as well as constraining dyadicity and heterophilicity within a specific range. Some computational experiences show the bounds' effectiveness and their usefulness with regards to different classes of networks

An empirical study of the Enterprise Europe Network

This article offers a network perspective on the collaborative effects of technology transfer, providing a research methodology based on the network science paradigm. We argue that such an approach is able to map and describe the set of entities acting in the technology transfer environment and their mutual relationships. We outline how the connections' patterns shape the organization of the networks by showing the role of the members within the system. By means of a case study of a transnational initiative aiming to support the technology transfer within European countries, we analyse the application of the network science approach, giving evidence of its relative implications

Measuring network resilience through connection patterns

Networks are at the core of modeling many engineering contexts, mainly in the case of infrastructures and communication systems. The resilience of a network, which is the property of the system capable of absorbing external shocks, is then of paramount relevance in the applications. This paper deals with this topic by advancing a theoretical proposal for measuring the resilience of a network. The proposal is based on the study of the shocks propagation along the patterns of connections among nodes. The theoretical model is tested on the real-world instances of two important airport systems in the US air traffic network; Illinois (including the hub of Chicago) and New York states (with JFK airport).Comment: Keywords: networks; resilience; paths; weighted arcs; air traffic system

Network-based principles of entrepreneurial ecosystems: a case study of a start-up network.

Entrepreneurial ecosystems are wealthy environments in which entrepreneurs, firms, and governments can operate frictionless, contributing to innovation and economic growth. The investigation of the structure of such systems is an open issue. We provide insights on this aspect through the formulation of seven network-based principles associating specific network metrics to distinct structural features of entrepreneurial ecosystems. In this way, we aim to support the measurement of the structural characteristics of an entrepreneurial ecosystem and the design of policy interventions in case of unmet properties. The proposed methodology is applied to an original network built on the relationships occurring on Twitter among 612 noteworthy start-ups from seven different European countries. This is a novel way to conceptualize entrepreneurial ecosystems considering online interactions. Thus, this work represents a first attempt to analyze the structure of entrepreneurial ecosystems considering their network architecture to guide policy-making decisions. Our results suggest a partial ecosystem-like nature of the analyzed network, providing evidence about possible policy recommendations

Quantitation of cellular deoxynucleoside triphosphates

Eukaryotic cells contain a delicate balance of minute amounts of the four deoxyribonucleoside triphosphates (dNTPs), sufficient only for a few minutes of DNA replication. Both a deficiency and a surplus of a single dNTP may result in increased mutation rates, faulty DNA repair or mitochondrial DNA depletion. dNTPs are usually quantified by an enzymatic assay in which incorporation of radioactive dATP (or radioactive dTTP in the assay for dATP) into specific synthetic oligonucleotides by a DNA polymerase is proportional to the concentration of the unknown dNTP. We find that the commonly used Klenow DNA polymerase may substitute the corresponding ribonucleotide for the unknown dNTP leading in some instances to a large overestimation of dNTPs. We now describe assay conditions for each dNTP that avoid ribonucleotide incorporation. For the dTTP and dATP assays it suffices to minimize the concentrations of the Klenow enzyme and of labeled dATP (or dTTP); for dCTP and dGTP we had to replace the Klenow enzyme with either the Taq DNA polymerase or Thermo Sequenase. We suggest that in some earlier reports ribonucleotide incorporation may have caused too high values for dGTP and dCTP

The Intention to Purchase Recycled Products: Towards an Integrative Theoretical Framework

The growing interest of the scientific literature regarding purchase behavior, circular economy and new business models has generated the need, as well as the opportunity, for a comprehensive review and categorization of the state of the existing research carried out so far. The present study aims at reconciling the wide but fragmented literature dealing with the purchase intention of recycled products. An integrative theoretical framework, able to combine several constructs, perspectives, and theories discussed to date on the topic, is proposed. Such framework represents a further step toward a comprehensive understanding of behavioral theories and constructs, which need to be understood to design effective business models for the circular economy. This effort could be highly valuable both for scholars interested in the topic—as the integrative framework could assist them in theorizing additional effects—and for firms’ managers—who can understand, more in depth, the drivers of the consumers’ purchasing process and act accordingly

p53R2-dependent ribonucleotide reduction provides deoxyribonucleotides in quiescent human fibroblasts in the absence of induced DNA damage.

Human fibroblasts in culture obtain deoxynucleotides by de novo ribonucleotide reduction or by salvage of deoxynucleosides. In cycling cells the de novo pathway dominates, but in quiescent cells the salvage pathway becomes important. Two forms of active mammalian ribonucleotide reductases are known. Each form contains the catalytic R1 protein, but the two differ with respect to the second protein (R2 or p53R2). R2 is cell cycle-regulated, degraded during mitosis, and absent from quiescent cells. The recently discovered p53-inducible p53R2 was proposed to be linked to DNA repair processes. The protein is not cell cycle-regulated and can provide deoxynucleotides to quiescent mouse fibroblasts. Here we investigate the in situ activities of the R1-p53R2 complex and two other enzymes of the de novo pathway, dCMP deaminase and thymidylate synthase, in confluent quiescent serum-starved human fibroblasts in experiments with [5-(3)H]cytidine, [6-(3)H]deoxycytidine, and [C(3)H(3)]thymidine. These cells had increased their content of p53R2 2-fold and lacked R2. From isotope incorporation, we conclude that they have a complete de novo pathway for deoxynucleotide synthesis, including thymidylate synthesis. During quiescence, incorporation of deoxynucleotides into DNA was very low. Deoxynucleotides were instead degraded to deoxynucleosides and exported into the medium as deoxycytidine, deoxyuridine, and thymidine. The rate of export was surprisingly high, 25% of that in cycling cells. Total ribonucleotide reduction in quiescent cells amounted to only 2-3% of cycling cells. We suggest that in quiescent cells an important function of p53R2 is to provide deoxynucleotides for mitochondrial DNA replication

Nucleotide excision repair efficiency in quiescent human fibroblasts is modulated by circadian clock

The efficiency of Nucleotide Excision Repair (NER)process is crucial for maintaining genomic integrity because in many organisms, including humans, it represents the only system able to repair a wide range of DNA damage. The aim of the work was to investigate whether the efficiency of the repair of photoproducts induced by UV-light is affected by the circadian phase at which irradiation occurred. NER activity has been analyzed in human quiescent fibroblasts (in the absence of the cell cycle effect), in which circadian rhythmicity has been synchronized with a pulse of dexamethasone. Our results demonstrate that both DNA damage induction and repair efficiency are strictly dependent on the phase of the circadian rhythm at which the cells are UV-exposed. Furthermore, the differences observed between fibroblasts irradiated at different circadian times (CTs) are abolished when the clock is obliterated. In addition, we observe that chromatin structure is regulated by circadian rhythmicity. Maximal chromatin relaxation occurred at the same CT when photoproduct formation and removal were highest. Our data suggest that the circadian clock regulates both the DNA sensitivity to UV damage and the efficiency of NER by controlling chromatin condensation mainly through histone acetylatio

Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in cultured cells and functional aspects.

Deoxynucleoside triphosphates (dNTPs) used for mitochondrial DNA replication are mainly formed by phosphorylation of deoxynucleosides imported into mitochondria from the cytosol. We earlier obtained evidence for a mitochondrial 5′-nucleotidase (dNT2) with a pronounced specificity for dUMP and dTMP and suggested that the enzyme protects mitochondrial DNA replication from excess dTTP. In humans, accumulation of dTTP causes a mitochondrial genetic disease. We now establish that dNT2 in vivo indeed is located in mitochondria. The native enzyme shows the same substrate specificity and affinity for inhibitors as the recombinant dNT2. We constructed ponasterone-inducible cell lines overproducing dNT2 with and without the green fluorescent protein (GFP) linked to its C terminus. The fusion protein occurred in mitochondria mostly in an inactive truncated form, with only a short C-terminal fragment of dNT2 linked to GFP. No truncation occurred when dNT2 and GFP were not linked. The cell mitochondria then contained a large excess of active dNT2 with or without the mitochondrial presequence. After removal of ponasterone overproduced dNT2 disappeared only slowly from the cells, whereas dNT2-mRNA was lost rapidly. Overproduction of dNT2 did not lead to an increased excretion of pyrimidine deoxyribonucleosides, in contrast to overproduction of the corresponding cytosolic deoxynucleotidase, suggesting that the mitochondrial enzyme does not affect overall cellular deoxynucleotide turnover